Plasmid

Part:BBa_K3394007:Design

Designed by: Talia Shaler   Group: iGEM20_BGU-Israel   (2020-10-17)


Cellulolytic complex [1] with AIDA-I


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
    Illegal EcoRI site found at 2117
    Illegal EcoRI site found at 2813
    Illegal EcoRI site found at 6015
    Illegal PstI site found at 2165
    Illegal PstI site found at 2751
    Illegal PstI site found at 5263
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
    Illegal EcoRI site found at 2117
    Illegal EcoRI site found at 2813
    Illegal EcoRI site found at 6015
    Illegal PstI site found at 2165
    Illegal PstI site found at 2751
    Illegal PstI site found at 5263
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
    Illegal EcoRI site found at 2117
    Illegal EcoRI site found at 2813
    Illegal EcoRI site found at 6015
    Illegal BamHI site found at 28
    Illegal BamHI site found at 3015
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
    Illegal EcoRI site found at 2117
    Illegal EcoRI site found at 2813
    Illegal EcoRI site found at 6015
    Illegal PstI site found at 2165
    Illegal PstI site found at 2751
    Illegal PstI site found at 5263
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
    Illegal EcoRI site found at 2117
    Illegal EcoRI site found at 2813
    Illegal EcoRI site found at 6015
    Illegal PstI site found at 2165
    Illegal PstI site found at 2751
    Illegal PstI site found at 5263
    Illegal NgoMIV site found at 3227
    Illegal NgoMIV site found at 3274
    Illegal NgoMIV site found at 3642
    Illegal NgoMIV site found at 3802
    Illegal NgoMIV site found at 4156
    Illegal AgeI site found at 1289
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5089


Design Notes

We designed the order of genes in the system.


Source

Artificial sequence - Synthetic construct

References

Jose, J., & Meyer, T. F. (2007). The Autodisplay Story, from Discovery to Biotechnical and Biomedical Applications. Microbiology and Molecular Biology Reviews, 71(4), 600–619.

Amir, L., Carnally, S. A., Rayo, J., Rosenne, S., Melamed Yerushalmi, S., Schlesinger, O., ... & Alfonta, L. (2013). Surface display of a redox enzyme and its site-specific wiring to gold electrodes. Journal of the American Chemical Society, 135(1), 70-73.